Midwest Mountain Lions
| # | Player | Position |
|---|---|---|
| 1 | Monica Jones | DB |
| 2 | Chloe McClain | DB |
| 6 | Mary Ellen Six | DB |
| 8 | Lily Galicia | DB |
| 12 | Cara Hamer | DL, LB |
| 13 | Kelly Rehr-Scarriot | DL |
| 14 | Danielle Tilseth | DB |
| 16 | Amaya Campbell | DB |
| 17 | Ava Matz | DB |
| 20 | Jennifer Poetker | DB |
| 21 | Michaela DeLuca | LB |
| 25 | Julie Sullivan | DL |
| 30 | Tiana Benjamin | DB |
| 32 | Bridgette Harris | LB |
| 42 | Michelle Simon | DB, LB |
| 53 | Hannah Koehlert | DL |
| 55 | Kamajjae Patterson | DL |
| 63 | Alisa Jungbluth | DL, LB |
| 64 | Katherine Deuberry | DL |
| 66 | Kaitlyn Wolfer | DL |
| 69 | Mary Kate Christensen | DL |
| 71 | Antoinette Harris | DL |
| 77 | Antoniesha Ward | DL |
| 87 | Jessica Powell | DL, LB |
| 89 | Hayley Syring | DL |
| 92 | Anaya Andrews | DL |
| 96 | Jennifer Mussa | DL |